2187-pT7A3HC-6H-HA2-R9

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Cloning of 2187-pT7A3HC-6H-HA2-R9

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Purpose: Cloning of 2187-pT7A3HC-6H-HA2-R9

Date: 2014-5-2---2014-5-5

Notebook:0

Page:

Clone ID:2187

Experimenters: yingying

Results

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cloning

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mix 4 oligos, heat, cool slowing to RT to anneal, then ligate to Nde1 + Xho1 digested 2021 pT7A3E-6H-HC

HA2-R9-1

HA2-R9-2

HA2-R9-3 

HA2-R9-4

Colony Screening

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Date: 2014-5-5

Make miniprep and PCR using
HA2-R9-3
and pET-seq-F



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Result intepretation/Summary

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Conclusion

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