2187-pT7A3HC-6H-HA2-R9
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Cloning of 2187-pT7A3HC-6H-HA2-R9
[edit]Purpose: Cloning of 2187-pT7A3HC-6H-HA2-R9
Date: 2014-5-2---2014-5-5
Notebook:0
Page:
Clone ID:2187
Experimenters: yingying
Results
[edit]cloning
[edit]
mix 4 oligos, heat, cool slowing to RT to anneal, then ligate to Nde1 + Xho1 digested 2021 pT7A3E-6H-HC
HA2-R9-1
HA2-R9-2
HA2-R9-3
HA2-R9-4
Colony Screening
[edit]Date: 2014-5-5
Make miniprep and PCR using
HA2-R9-3
and pET-seq-F
<br>
Result intepretation/Summary
[edit]Conclusion
[edit]