2180-pT7A3-R9-HA-iFlp

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Cloning of 2180-pT7A3-R9-HA-iFlp

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Purpose: Cloning of 2180-pT7A3-R9-HA-iFlp

Date: 2014-4-6---2014-4-9

Notebook:0

Page:

Clone ID:2180

Experimenters: yingying

Results

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cloning

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R9-HA-1 to R9-HA-4


lane
 7
 8

 pcr
 2115 pT7A3-iFLP

 Nco1+ HindIII


Colony Screening

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Date: 2014-4-9

Make miniprep and digest with Nco1+ HindIII


c:1823 pCold-HTNCre


Date: 2014-5-12

Pick 6 more clones, make miniprep and digest with Nco1+ HindIII


c:1823 pCold-HTNCre

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Result intepretation/Summary

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Conclusion

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