2180-pT7A3-R9-HA-iFlp
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Cloning of 2180-pT7A3-R9-HA-iFlp
[edit]Purpose: Cloning of 2180-pT7A3-R9-HA-iFlp
Date: 2014-4-6---2014-4-9
Notebook:0
Page:
Clone ID:2180
Experimenters: yingying
Results
[edit]cloning
[edit]
R9-HA-1 to R9-HA-4
| lane |
7 |
8 |
| pcr |
2115 pT7A3-iFLP | |
| Nco1+ HindIII | ||
Colony Screening
[edit]Date: 2014-4-9
Make miniprep and digest with Nco1+ HindIII
c:1823 pCold-HTNCre
Date: 2014-5-12
Pick 6 more clones, make miniprep and digest with Nco1+ HindIII
c:1823 pCold-HTNCre
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Result intepretation/Summary
[edit]Conclusion
[edit]