444 pT7A3E-SumoProtease
Cloning of444 pT7A3E-SumoProtease
[edit]Purpose: Cloning of 444 pT7A3E-SumoProtease
Date: 2014-5-
Notebook:0
Page:
Clone ID:444
Experimenters: Shanyi
Results
[edit]PCR
[edit]PCR from yeast (for making bread) genomic DNA
Fragment 1
Sumo-Nde1-F
tagcac catatgtcagttgaagtagataag
Sumo-R1
cacgtagaccataCggtacgtc
Fragment 2
Sumo-F1
gacgtaccGtatggtctacgtg
Sumo-Xho1-R
(gattttaaccgacgctttaaaactcgag tagcac)
gtgctactcgagttttaaagcgtcggttaaaatc
Join two fragments using
Sumo-Nde1-F
tagcac catatgtcagttgaagtagataag
Sumo-Xho1-R
(gattttaaccgacgctttaaaactcgag tagcac)
gtgctactcgagttttaaagcgtcggttaaaatc
digest with Nde1 + Xho1,CLONE TO 1892
Colony Screening
[edit]Date: 2013-2-
digest with Nde1 + Xho1
Result intepretation/Summary
[edit]- <br>
Conclusion
[edit]