444 pT7A3E-SumoProtease

Cloning of444 pT7A3E-SumoProtease

Purpose: Cloning of 444 pT7A3E-SumoProtease

Date: 2014-5-

Notebook:0

Page:

Clone ID:444

Experimenters: Shanyi

Results

PCR

PCR from yeast (for making bread) genomic DNA

Fragment 1

Sumo-Nde1-F
tagcac catatgtcagttgaagtagataag
Sumo-R1
cacgtagaccataCggtacgtc

Fragment 2
Sumo-F1
gacgtaccGtatggtctacgtg
Sumo-Xho1-R
(gattttaaccgacgctttaaaactcgag tagcac)
gtgctactcgagttttaaagcgtcggttaaaatc

Join two fragments using

Sumo-Nde1-F
tagcac catatgtcagttgaagtagataag
Sumo-Xho1-R
(gattttaaccgacgctttaaaactcgag tagcac)
gtgctactcgagttttaaagcgtcggttaaaatc

digest with Nde1 + Xho1,CLONE TO 1892

Colony Screening

Date: 2013-2-

digest with Nde1 + Xho1

Result intepretation/Summary

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