1403-pCDNA3.1-NLS-Cre

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Cloning of 1403-pCDNA3.1-NLS-Cre

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Purpose: Cloning of 1403-pCDNA3.1-NLS-Cre

Date: 2012-9-25---2012-9-27

Notebook:0

Page:

Clone ID:1403

Experimenters: yingying

Results

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subcloning

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subclone from 241 pLentiGP-Cre using EcoR1 + Xho1 to 1226 pCDNA3.1+


Colony Screening

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Date: 2012-9-27

mini prep and digest with EcoR1 + Xho1


clone 3 and 6 are right

1403 digest with EcoRI+XhoI


1403DIGEST WITH DraIII +EcoRI(1), EcoRI+XbaI(2)

1403DIGEST WITH DraIII +EcoRI, should get  two bands, if it is 241, should get there bands.

1403DIGEST WITH EcoRI+XbaI,should get  two bands, if it is 241,only one.




Cloning of 1403-pCDNA3.1-NLS-Cre

[edit]

Purpose: Cloning of 1403-pCDNA3.1-NLS-Cre

Date: 2014-6

Notebook:0

Page:

Clone ID:1403

Experimenters: shanyi

Results

[edit]

subcloning

[edit]

subclone from 241 pLentiGP-Cre using EcoR1 + Xho1 to 1226 pCDNA3.1+


Colony Screening

[edit]

Date: 2014-6

mini prep and digest with EcoR1 + Xho1

verify


Result intepretation/Summary

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  • <br>

Conclusion

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<br>





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