1403-pCDNA3.1-NLS-Cre
Cloning of 1403-pCDNA3.1-NLS-Cre
[edit]Purpose: Cloning of 1403-pCDNA3.1-NLS-Cre
Date: 2012-9-25---2012-9-27
Notebook:0
Page:
Clone ID:1403
Experimenters: yingying
Results
[edit]subcloning
[edit]
subclone from 241 pLentiGP-Cre using EcoR1 + Xho1 to 1226 pCDNA3.1+
Colony Screening
[edit]Date: 2012-9-27
mini prep and digest with EcoR1 + Xho1
clone 3 and 6 are right
1403 digest with EcoRI+XhoI
1403DIGEST WITH DraIII +EcoRI(1), EcoRI+XbaI(2)
1403DIGEST WITH DraIII +EcoRI, should get two bands, if it is 241, should get there bands.
1403DIGEST WITH EcoRI+XbaI,should get two bands, if it is 241,only one.
Cloning of 1403-pCDNA3.1-NLS-Cre
[edit]Purpose: Cloning of 1403-pCDNA3.1-NLS-Cre
Date: 2014-6
Notebook:0
Page:
Clone ID:1403
Experimenters: shanyi
Results
[edit]subcloning
[edit]subclone from 241 pLentiGP-Cre using EcoR1 + Xho1 to 1226 pCDNA3.1+
 |
Colony Screening
[edit]Date: 2014-6
mini prep and digest with EcoR1 + Xho1
verify
Result intepretation/Summary
[edit]- <br>
Conclusion
[edit]<br>