2099 pFastBac-Dual-VSVG-HIV-Gag-Cre

From ELN
Revision as of 14:23, 12 June 2014 by Paul.xu (talk | contribs) (Created page with "== '''Cloning of 2099 pFastBac-Dual-VSVG-HIV-Gag-Cre''' == '''Purpose:''' Cloning of 2099 pFastBac-Dual-VSVG-HIV-Gag-Cre '''Date: '''2014-5 '''Notebook:0''' '''Page:''' ...")
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)
Jump to navigation Jump to search

Cloning of 2099 pFastBac-Dual-VSVG-HIV-Gag-Cre

[edit]

Purpose: Cloning of 2099 pFastBac-Dual-VSVG-HIV-Gag-Cre

Date: 2014-5

Notebook:0

Page:

Clone ID:2099

Experimenters: shanyi

Results

[edit]

subcloning

[edit]

subclone from 1968 pCMV5-Gag-p2b-NLS-Cre-WPRE-oriPusing EcoRI + Xba1 to 2226 pFastBac-Dual-VSVG-HIVGag-FLP




Colony Screening

[edit]

Date: 2014-6

mini prep and digest with BamH1



verify using Not1 + Xba1




Result intepretation/Summary

[edit]
  • <br>

Conclusion

[edit]

<br>





Retrieved from "https://eln.excellgen.com/index.php?title=2099_pFastBac-Dual-VSVG-HIV-Gag-Cre&oldid=11975"