2099 pFastBac-Dual-VSVG-HIV-Gag-Cre

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Cloning of 2099 pFastBac-Dual-VSVG-HIV-Gag-Cre

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Purpose: Cloning of 2099 pFastBac-Dual-VSVG-HIV-Gag-Cre

Date: 2014-5

Notebook:0

Page:

Clone ID:2099

Experimenters: shanyi

Results

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subcloning

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subclone from 1968 pCMV5-Gag-p2b-NLS-Cre-WPRE-oriPusing EcoRI + Xba1 to 2226 pFastBac-Dual-VSVG-HIVGag-FLP




Colony Screening

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Date: 2014-6

mini prep and digest with BamH1



verify using Not1 + Xba1




Result intepretation/Summary

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  • <br>

Conclusion

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<br>