2221 pFastbacDual-p10-VSVG-M-6H-Tat-Cre-MFGE8

From ELN

Revision as of 14:41, 24 June 2014 by Excellgen (talk | contribs) (Created page with "== '''Cloning of 2221 pFastbacDual-p10-VSVG-M-6H-Tat-Cre-MFGE8''' == '''Purpose:''' Cloning of 2221 pFastbacDual-p10-VSVG-M-6H-Tat-Cre-MFGE8'''==''' '''Date: '''2014-6---- ...")

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

Jump to navigation Jump to search

Cloning of 2221 pFastbacDual-p10-VSVG-M-6H-Tat-Cre-MFGE8

Purpose: Cloning of 2221 pFastbacDual-p10-VSVG-M-6H-Tat-Cre-MFGE8==

Date: 2014-6----

Notebook:0

Page:

Clone ID:2221

Experimenters: shanyi

Results

CLONING

subclone 6H-Tat-Cre-MFGE8 from 2123 pCMV5-Mfge8-Tat-Cre-MFGE8 to 2224 pFastbacDual-p10-VSVG-M

Colony Screening

Date: 2014

Make miniprep, dDigestion with BamH1 to screen,

digest positive clone with Nhe1 + Not1 to confirm

Result intepretation/Summary

Conclusion

Retrieved from "https://eln.excellgen.com/index.php?title=2221_pFastbacDual-p10-VSVG-M-6H-Tat-Cre-MFGE8&oldid=12101"

Cloning