2221 pFastbacDual-p10-VSVG-M-6H-Tat-Cre-MFGE8
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Cloning of 2221 pFastbacDual-p10-VSVG-M-6H-Tat-Cre-MFGE8
[edit]Purpose: Cloning of 2221 pFastbacDual-p10-VSVG-M-6H-Tat-Cre-MFGE8==
Date: 2014-6----
Notebook:0
Page:
Clone ID:2221
Experimenters: shanyi
Results
[edit]CLONING
[edit]subclone 6H-Tat-Cre-MFGE8 from 2123 pCMV5-Mfge8-Tat-Cre-MFGE8 to 2224 pFastbacDual-p10-VSVG-M
Colony Screening
[edit]Date: 2014
Make miniprep, dDigestion with BamH1 to screen,
digest positive clone with Nhe1 + Not1 to confirm