2219 pFastbacDual-p10-VSVG-M-6H-Tat-Flp-MFGE8

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Cloning of 2219 pFastbacDual-p10-VSVG-M-6H-Tat-Flp-MFGE8

Purpose: Cloning of 2219 pFastbacDual-p10-VSVG-M-6H-Tat-Flp-MFGE8==

Date: 2014-6----

Notebook:0

Page:

Clone ID:2219

Experimenters: shanyi

Results

CLONING

subclone 6H-Tat-FLP-MFGE8 from 2122 pCMV5-Mfge8-Tat-FLP-MFGE8 to 2224 pFastbacDual-p10-VSVG-M using Nhe1 + Not1

Colony Screening

Date: 2014

Make miniprep, dDigestion with BamH1 to screen,

digest positive clone with Nhe1 + Not1 to confirm

Result intepretation/Summary

Conclusion

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Cloning