2219 pFastbacDual-p10-VSVG-M-6H-Tat-Flp-MFGE8

From ELN
Jump to navigation Jump to search

Cloning of 2219 pFastbacDual-p10-VSVG-M-6H-Tat-Flp-MFGE8

[edit]

Purpose: Cloning of 2219 pFastbacDual-p10-VSVG-M-6H-Tat-Flp-MFGE8==

Date: 2014-6----

Notebook:0

Page:

Clone ID:2219

Experimenters:  shanyi

Results

[edit]

CLONING

[edit]

subclone 6H-Tat-FLP-MFGE8 from 2122 pCMV5-Mfge8-Tat-FLP-MFGE8 to 2224 pFastbacDual-p10-VSVG-M using Nhe1 + Not1


Colony Screening

[edit]

Date: 2014

Make miniprep, dDigestion with BamH1 to screen,

digest positive clone with Nhe1 + Not1 to confirm

Result intepretation/Summary

[edit]

Conclusion

[edit]
Retrieved from "https://eln.excellgen.com/index.php?title=2219_pFastbacDual-p10-VSVG-M-6H-Tat-Flp-MFGE8&oldid=12102"