2248-pT7A3E-UDG

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Revision as of 15:16, 7 July 2014 by Excellgen (talk | contribs) (Created page with "== '''Cloning of 2248-pT7A3E-UDG''' == '''Purpose:''' Cloning of 2248-pT7A3E-ULP1 '''Date: '''2014-6-20---2014-6-26 '''Notebook:0''' '''Page:''' '''Clone ID:2248''' '...")
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Cloning of 2248-pT7A3E-UDG

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Purpose: Cloning of 2248-pT7A3E-ULP1

Date: 2014-6-20---2014-6-26

Notebook:0

Page:

Clone ID:2248

Experimenters: LIANGHUO

Results

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cloning

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PCR using


UDG-Nde1-F
tacaccatATGGAACTGTTTGATGAACAG


UDG-Xho1-R
(GATTGATTGGCAACTGCCGCAG ctcgag tatag)



2248 DIGESTED

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Colony Screening

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Date: 2014-6-26

Make miniprep and digest with Nde1 + XhoI



SELECT CLONE1 FOR SEQUENCING



<br>

Result intepretation/Summary

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Conclusion

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