2248-pT7A3E-UDG
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Cloning of 2248-pT7A3E-UDG
[edit]Purpose: Cloning of 2248-pT7A3E-ULP1
Date: 2014-6-20---2014-6-26
Notebook:0
Page:
Clone ID:2248
Experimenters: LIANGHUO
Results
[edit]cloning
[edit]
PCR using
UDG-Nde1-F
tacaccatATGGAACTGTTTGATGAACAG
UDG-Xho1-R
(GATTGATTGGCAACTGCCGCAG ctcgag tatag)
2248 DIGESTED
[edit]
Colony Screening
[edit]Date: 2014-6-26
Make miniprep and digest with Nde1 + XhoI
SELECT CLONE1 FOR SEQUENCING
<br>
Result intepretation/Summary
[edit]Conclusion
[edit]