2212-pT7A3E-PDI1

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Cloning of 2212-pT7A3E-PDI1

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Purpose: Cloning of 2212-pT7A3E-PDI1

Date: 2014-7-01---2014-7-02

Notebook:0

Page:

Clone ID:2212

Experimenters: LIANGHUO

Results

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cloning

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PCR from 2208 pDONR221-PDI1

yPDI-Nde1-F
tcgaacatATGAAGTTTTCTGCTGGTGC

yPDI-Xho1-R
(GATGCCATTCACGATGAATTGctcgag tagca)
tgctactcgagCAATTCATCGTGAATGGCATC


PCR image?

2212 DIGESTED

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Colony Screening

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Date: 2014-7-01

Make miniprep and digest with NdeI + XhoI



SELECT CLONE2 FOR SEQUENCING



<br>

Result intepretation/Summary

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Conclusion

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