2212-pT7A3E-PDI1
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Cloning of 2212-pT7A3E-PDI1
[edit]Purpose: Cloning of 2212-pT7A3E-PDI1
Date: 2014-7-01---2014-7-02
Notebook:0
Page:
Clone ID:2212
Experimenters: LIANGHUO
Results
[edit]cloning
[edit]PCR from 2208 pDONR221-PDI1
yPDI-Nde1-F
tcgaacatATGAAGTTTTCTGCTGGTGC
yPDI-Xho1-R
(GATGCCATTCACGATGAATTGctcgag tagca)
tgctactcgagCAATTCATCGTGAATGGCATC
PCR image?
2212 DIGESTED
[edit]
Colony Screening
[edit]Date: 2014-7-01
Make miniprep and digest with NdeI + XhoI
SELECT CLONE2 FOR SEQUENCING
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Result intepretation/Summary
[edit]Conclusion
[edit]