2242-pT7A3E-T4-GP32

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Cloning of 2242-pT7A3E-T4-GP32

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Purpose: Cloning of 2242-pT7A3E-T4-GP32

Date: 2014-7-01---2014-7-02

Notebook:0

Page:

Clone ID:2242

Experimenters: LIANGHUO

Results

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cloning

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PCR using
T4_G32-Nde1-F2 acgata CATATG tttaaacgtaaatctactgc

T4_G32-Xho1-R2
(gatgaccttttgaatgacctt CTCGAG cgagca)
tgctcg CTCGAG aaggtcattcaaaaggtcatc



2242 DIGESTED

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Colony Screening

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Date: 2014-7-02

Make miniprep and digest with Nde1 + XhoI



SELECT CLONE3 FOR SEQUENCING



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Result intepretation/Summary

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Conclusion

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