2242-pT7A3E-T4-GP32
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Cloning of 2242-pT7A3E-T4-GP32
[edit]Purpose: Cloning of 2242-pT7A3E-T4-GP32
Date: 2014-7-01---2014-7-02
Notebook:0
Page:
Clone ID:2242
Experimenters: LIANGHUO
Results
[edit]cloning
[edit]PCR using
T4_G32-Nde1-F2 acgata CATATG tttaaacgtaaatctactgc
T4_G32-Xho1-R2
(gatgaccttttgaatgacctt CTCGAG cgagca)
tgctcg CTCGAG aaggtcattcaaaaggtcatc
2242 DIGESTED
[edit]
Colony Screening
[edit]Date: 2014-7-02
Make miniprep and digest with Nde1 + XhoI
SELECT CLONE3 FOR SEQUENCING
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Result intepretation/Summary
[edit]Conclusion
[edit]