Synthesis of Pullulan-PEI

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Dissolve pullulan in DMSO, stire for 0.5 hour.

Add 6 mM discuccinmidyl carbonate while stirring

Add 6 mM dimethylaminopyridine while stirring

React at RT (25 oC) for 5 hour.

Cool to 4 oC

add PEI in weight ratios 1:0.25 (PEI is 0.25).

React o/n (16 hours)

Precipitate PEI-Pullulan using acetone.

Wash 5 times with acetone to remove unreacted PEI

Dry at 37 oC



 

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