734 pLike-rep-Not1

From ELN
Revision as of 16:13, 28 July 2014 by Excellgen (talk | contribs)
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)
Jump to navigation Jump to search

Cloning of 734 pLike-rep-Not1

[edit]

Purpose: Cloning of 734pLike-rep-Not1==

Date: 2014-7----

Notebook:0

Page:

Clone ID:734

Experimenters:  shanyi

Results

[edit]

CLONING

[edit]

PCR from 567
Not1-Xba1-F
atacactctagaTGCGTAGCGATACCAGCGTTG

Not1-HindIII-R
(CAGCGTGGTCTGTTTGATGCA CACCACCACCACCACCACTGA AAGCTT)
AAGCTTTCAGTGGTGGTGGTGGTGGTGTGCATCAAACAGACCACGCTG



digest with Xba1 +HindIII, clone to 2270 pLIKE-rep




Colony Screening

[edit]

Date: 2014_2_14

Make miniprep, use  Xba1 +HindIII to find positive clone






Result intepretation/Summary

[edit]

Conclusion

[edit]
Retrieved from "https://eln.excellgen.com/index.php?title=734_pLike-rep-Not1&oldid=12571"