734 pLike-rep-Not1

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Cloning of 734 pLike-rep-Not1

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Purpose: Cloning of 734pLike-rep-Not1==

Date: 2014-7----

Notebook:0

Page:

Clone ID:734

Experimenters:  shanyi

Results

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CLONING

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PCR from 567
Not1-Xba1-F
atacactctagaTGCGTAGCGATACCAGCGTTG

Not1-HindIII-R
(CAGCGTGGTCTGTTTGATGCA CACCACCACCACCACCACTGA AAGCTT)
AAGCTTTCAGTGGTGGTGGTGGTGGTGTGCATCAAACAGACCACGCTG



digest with Xba1 +HindIII, clone to 2270 pLIKE-rep




Colony Screening

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Date: 2014_2_14

Make miniprep, use  Xba1 +HindIII to find positive clone






Result intepretation/Summary

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Conclusion

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