1575 pT7A3E-RecJ

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Cloning of 1575 pT7A3E-RecJ

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Purpose: Cloning of1575 pT7A3E-RecJ==

Date: 2014-7----

Notebook:0

Page:

Clone ID:1575

Experimenters:  shanyi

Results

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CLONING

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PCR from MG1655

PCR 1st fragment
RecJ-Nde1-F tagcacatatgaaacaacagatacaac
RecJ-R1 catcacgcatGtgcagcccct


PCR 2nd fragment
RecJ-F1 aggggctgcaCatgcgtgatg
RecJ-Xho1-R gtcgactcgagaattggccagatattgtcgatg



Join 2 fragments
RecJ-Nde1-F tagcacatatgaaacaacagatacaac
RecJ-Xho1-R gtcgactcgagaattggccagatattgtcgatg


Digest with Nde1+XhoI Clone into 1892r



Colony Screening

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Date: 2014_7

Make miniprep, use Nde1+XhoI to find positive clone





Result intepretation/Summary

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Conclusion

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