1575 pT7A3E-RecJ
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Cloning of 1575 pT7A3E-RecJ
[edit]Purpose: Cloning of1575 pT7A3E-RecJ==
Date: 2014-7----
Notebook:0
Page:
Clone ID:1575
Experimenters: shanyi
Results
[edit]CLONING
[edit]PCR from MG1655
PCR 1st fragment
RecJ-Nde1-F tagcacatatgaaacaacagatacaac
RecJ-R1 catcacgcatGtgcagcccct
PCR 2nd fragment
RecJ-F1 aggggctgcaCatgcgtgatg
RecJ-Xho1-R gtcgactcgagaattggccagatattgtcgatg
Join 2 fragments
RecJ-Nde1-F tagcacatatgaaacaacagatacaac
RecJ-Xho1-R gtcgactcgagaattggccagatattgtcgatg
Digest with Nde1+XhoI Clone into 1892r
Colony Screening
[edit]Date: 2014_7
Make miniprep, use Nde1+XhoI to find positive clone