2289 pT7A30-SMT3

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Cloning of 2289 pT7A30-SMT3

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Purpose: Cloning of2289 pT7A30-SMT3==

Date: 2014-7----

Notebook:0

Page:

Clone ID:2289

Experimenters:  shanyi

Results

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CLONING

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PCR from 2285 pBY011-SMT3
MT3-F Nde1
Sequence atcg CATATG TCGGACTCAGAAGTCAATC
Year 2010
Date 1/13/2010

SMT3-R Nco1
Sequence cat ccatggcACCACCAATCTGTTCTCTGTG
Year 2010
Date 1/13/2010




Digest with Nde1+NcoI Clone into 1727



Colony Screening

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Date: 2014_7

Make miniprep, use Nde1+NcoIto find positive clone





Result intepretation/Summary

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Conclusion

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