2289 pT7A30-SMT3
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Cloning of 2289 pT7A30-SMT3
[edit]Purpose: Cloning of2289 pT7A30-SMT3==
Date: 2014-7----
Notebook:0
Page:
Clone ID:2289
Experimenters: shanyi
Results
[edit]CLONING
[edit]PCR from 2285 pBY011-SMT3
MT3-F Nde1
Sequence atcg CATATG TCGGACTCAGAAGTCAATC
Year 2010
Date 1/13/2010
SMT3-R Nco1
Sequence cat ccatggcACCACCAATCTGTTCTCTGTG
Year 2010
Date 1/13/2010
Digest with Nde1+NcoI Clone into 1727
Colony Screening
[edit]Date: 2014_7
Make miniprep, use Nde1+NcoIto find positive clone
