2288 pT7A3E-6H-HC-SMT3

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Cloning of 2288 pT7A3E-6H-HC-SMT3

Purpose: Cloning of2288 pT7A3E-6H-HC-SMT3==

Date: 2014-7----

Notebook:0

Page:

Clone ID:2288

Experimenters: shanyi

Results

CLONING

PCR from 2285 pBY011-SMT3
SMT3-F Nde1
Sequence atcg CATATG TCGGACTCAGAAGTCAATC
2288-SUMO-Xho1-R tgctactcgagttaACCACCAATCTGTTCTCTGTG

Digest with Nde1+XhoI Clone into 2021

Colony Screening

Date: 2014_7

Make miniprep, use Nde1+XhoI to find positive clone

Result intepretation/Summary

Conclusion

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Cloning