2288 pT7A3E-6H-HC-SMT3
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Cloning of 2288 pT7A3E-6H-HC-SMT3
[edit]Purpose: Cloning of2288 pT7A3E-6H-HC-SMT3==
Date: 2014-7----
Notebook:0
Page:
Clone ID:2288
Experimenters: shanyi
Results
[edit]CLONING
[edit]PCR from 2285 pBY011-SMT3
SMT3-F Nde1
Sequence atcg CATATG TCGGACTCAGAAGTCAATC
2288-SUMO-Xho1-R tgctactcgagttaACCACCAATCTGTTCTCTGTG
Digest with Nde1+XhoI Clone into 2021
Colony Screening
[edit]Date: 2014_7
Make miniprep, use Nde1+XhoI to find positive clone
