2233 pCMV5-Dest-C-HA

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Revision as of 15:13, 8 August 2014 by Excellgen (talk | contribs) (Created page with "== '''Cloning of 2233 pCMV5-Dest-C-HA''' == '''Purpose:''' Cloning of2233 pCMV5-Dest-C-HA'''==''' '''Date: '''2014-8---- '''Notebook:0''' '''Page:''' '''Clone ID:2233''...")
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Cloning of 2233 pCMV5-Dest-C-HA

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Purpose: Cloning of2233 pCMV5-Dest-C-HA==

Date: 2014-8----

Notebook:0

Page:

Clone ID:2233

Experimenters:  shanyi

Results

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CLONING

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PCR Gateway cassette from 1336

attR1-Fix-BamH1-F tagca ggatcc ATC ACA AGT TTG TAC AAA AAA G

attR2-HA-Nhe1-EcoR1-R (ctttcttgtacaaagtggt TAC CCA TAC GAT GTT CCA GAT TAC GCT taa GCTAGC gaattc ATAAAgaattcGCTAGCttaAGCGTAATCTGGAACATCGTATGGGTAaccactttgtacaagaaag digest with BamH1 + EcoR1

digest with BamH1 + EcoR1, clone into BamH1 + EcoR1 digested vector 1969




Colony Screening

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Date: 2014_7

Make miniprep, use BamH1 + EcoR1to find positive clone


Result intepretation/Summary

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Conclusion

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