2233 pCMV5-Dest-C-HA
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Cloning of 2233 pCMV5-Dest-C-HA
[edit]Purpose: Cloning of2233 pCMV5-Dest-C-HA==
Date: 2014-8----
Notebook:0
Page:
Clone ID:2233
Experimenters: shanyi
Results
[edit]CLONING
[edit]PCR Gateway cassette from 1336
attR1-Fix-BamH1-F tagca ggatcc ATC ACA AGT TTG TAC AAA AAA G
attR2-HA-Nhe1-EcoR1-R (ctttcttgtacaaagtggt TAC CCA TAC GAT GTT CCA GAT TAC GCT taa GCTAGC gaattc ATAAAgaattcGCTAGCttaAGCGTAATCTGGAACATCGTATGGGTAaccactttgtacaagaaag digest with BamH1 + EcoR1 
digest with BamH1 + EcoR1, clone into BamH1 + EcoR1 digested vector 1969
Colony Screening
[edit]Date: 2014_7
Make miniprep, use BamH1 + EcoR1to find positive clone
Result intepretation/Summary
[edit]Conclusion
[edit]