2201 pCMV5-Dest-Avi-8H

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Cloning of 2201 pCMV5-Dest-Avi-8H

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Purpose: Cloning of2201 pCMV5-Dest-Avi-8H==

Date: 2014-8----

Notebook:0

Page:

Clone ID:2201

Experimenters:  shanyi

Results

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CLONING

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PCR gateway cassette from 1336

1 ul of 10 uM attR1-Fix-BamH1-Nhe1-F attR1 tagca ggatcc GTGTC gct agc ATC ACA AGT TTG TAC AAA AAA G

1ul of 1 uM attR2-AviTag-R2 ( ctttcttgtacaaagtggtGGCGGCCTGAACGACATCTTCGAGGCCCAGAAAATCGAGTGGCAC) GTGCCACTCGATTTTCTGGGCCTCGAAGATGTCGTTCAGGCCGCCaccactttgtacaagaaag
1 ul of 10 uM attR2-AviTag8H-EcoR1-R1 (CAGAAAATCGAGTGGCACGAG CACCACCATCATCACCATCACCAC TGA GAATTC TTTAT) ATAAAGAATTC TCAGTGGTGATGGTGATGATGGTGGTGCTCGTGCCACTCGATTTTCTG


digest with BamH1 + EcoR1, clone into BamH1 + EcoR1 digested vector 1969




Colony Screening

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Date: 2014_7

Make miniprep, use BamH1 + EcoR1to find positive clone


Result intepretation/Summary

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Conclusion

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