2201 pCMV5-Dest-Avi-8H
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Cloning of 2201 pCMV5-Dest-Avi-8H
[edit]Purpose: Cloning of2201 pCMV5-Dest-Avi-8H==
Date: 2014-8----
Notebook:0
Page:
Clone ID:2201
Experimenters: shanyi
Results
[edit]CLONING
[edit]PCR gateway cassette from 1336
1 ul of 10 uM attR1-Fix-BamH1-Nhe1-F attR1 tagca ggatcc GTGTC gct agc ATC ACA AGT TTG TAC AAA AAA G
1ul of 1 uM attR2-AviTag-R2 ( ctttcttgtacaaagtggtGGCGGCCTGAACGACATCTTCGAGGCCCAGAAAATCGAGTGGCAC) GTGCCACTCGATTTTCTGGGCCTCGAAGATGTCGTTCAGGCCGCCaccactttgtacaagaaag
1 ul of 10 uM attR2-AviTag8H-EcoR1-R1 (CAGAAAATCGAGTGGCACGAG CACCACCATCATCACCATCACCAC TGA GAATTC TTTAT) ATAAAGAATTC TCAGTGGTGATGGTGATGATGGTGGTGCTCGTGCCACTCGATTTTCTG
digest with BamH1 + EcoR1, clone into BamH1 + EcoR1 digested vector 1969
Colony Screening
[edit]Date: 2014_7
Make miniprep, use BamH1 + EcoR1to find positive clone
Result intepretation/Summary
[edit]Conclusion
[edit]