2332 pT7A30-Cas9

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Cloning of 2332 pT7A30-Cas9

Purpose: Cloning of2332 pT7A30-Cas9==

Date: 2014-9----

Notebook:0

Page:

Clone ID:2332

Experimenters: shanyi

Results

CLONING

Subclone from 2275 CAS9P using Nco1 + Xho1 to 1727

Colony Screening

Date: 2014_9

Make miniprep, use Nde1 + Xho1 to find positive clone

digest with Nde1 + BamH1to verify

Result intepretation/Summary

Conclusion

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Cloning