2332 pT7A30-Cas9

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Cloning of 2332 pT7A30-Cas9

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Purpose: Cloning of2332 pT7A30-Cas9==

Date: 2014-9----

Notebook:0

Page:

Clone ID:2332

Experimenters:  shanyi

Results

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CLONING

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Subclone from 2275 CAS9P using Nco1 + Xho1 to 1727



Colony Screening

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Date: 2014_9

Make miniprep, use Nde1 + Xho1 to find positive clone


digest with Nde1 + BamH1to verify







Result intepretation/Summary

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Conclusion

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