2166 pT7A3E-T4-endo-VII

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Cloning of 2166 pT7A3E-T4-endo-VII

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Purpose: Cloning of2166 pT7A3E-T4-endo-VII==

Date: 2014-8----

Notebook:0

Page:

Clone ID:2166

Experimenters:  shanyi

Results

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CLONING

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PCR from T4 Genomic DNA

1st fragment

T4-endo-VII-Nde1-F atcga catatgttattgactggcaaattatac

T4-endo-VII-R1 caacaccttgtccctttaagccagaacg

2nd fragment

T4-endo-VII-F1cgttctggcttaaagggacaaggtgttg

T4-endo-VII-Xho1-R2 (cttagaaagagtttaaaa ctcgag ctagca) tgctagctcgagttttaaactctttctaag

join two fragments

T4-endo-VII-Nde1-F atcga catatgttattgactggcaaattatac

T4-endo-VII-Xho1-R2 (cttagaaagagtttaaaa ctcgag ctagca) tgctagctcgagttttaaactctttctaag


clone into 1892 using Nde1 +Xho1



Colony Screening

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Date: 2014_7

Make miniprep, use Nde1 +Xho1 to find positive clone


Cloning of 2166 pT7A3E-T4-endo-VII

[edit]

Purpose: Cloning of2166 pT7A3E-T4-endo-VII==

Date: 2014-9----

Notebook:0

Page:

Clone ID:2267

Experimenters:  shanyi

Results

[edit]

CLONING

[edit]

PCR from 1562


digest with Nde1 + Xho1




Colony Screening

[edit]

Date: 2014_8

Make miniprep, use  Nde1 + Xho1to find positive clone




Result intepretation/Summary

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Conclusion

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Result intepretation/Summary

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Conclusion

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