2166 pT7A3E-T4-endo-VII
Cloning of 2166 pT7A3E-T4-endo-VII
[edit]Purpose: Cloning of2166 pT7A3E-T4-endo-VII==
Date: 2014-8----
Notebook:0
Page:
Clone ID:2166
Experimenters: shanyi
Results
[edit]CLONING
[edit]PCR from T4 Genomic DNA
1st fragment
T4-endo-VII-Nde1-F atcga catatgttattgactggcaaattatac
T4-endo-VII-R1 caacaccttgtccctttaagccagaacg
2nd fragment
T4-endo-VII-F1cgttctggcttaaagggacaaggtgttg
T4-endo-VII-Xho1-R2 (cttagaaagagtttaaaa ctcgag ctagca) tgctagctcgagttttaaactctttctaag
join two fragments
T4-endo-VII-Nde1-F atcga catatgttattgactggcaaattatac
T4-endo-VII-Xho1-R2 (cttagaaagagtttaaaa ctcgag ctagca) tgctagctcgagttttaaactctttctaag
clone into 1892 using Nde1 +Xho1
Colony Screening
[edit]Date: 2014_7
Make miniprep, use Nde1 +Xho1 to find positive clone
Cloning of 2166 pT7A3E-T4-endo-VII
[edit]Purpose: Cloning of2166 pT7A3E-T4-endo-VII==
Date: 2014-9----
Notebook:0
Page:
Clone ID:2267
Experimenters: shanyi
Results
[edit]CLONING
[edit]PCR from 1562
digest with Nde1 + Xho1
Colony Screening
[edit]Date: 2014_8
Make miniprep, use Nde1 + Xho1to find positive clone
Result intepretation/Summary
[edit]Conclusion
[edit]Result intepretation/Summary
[edit]Conclusion
[edit]