2331 pT7A31

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Cloning of 2331 pT7A31

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Purpose: Cloning of2331 pT7A31==

Date: 2014-9-15---

Notebook:0

Page:

Clone ID:2331

Experimenters:  shanyi

Results

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CLONING

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digest vector with Nde1 + Nco1.


Ligate to annealed oligos 2331-Nde1-6His-Nco1-T + 2331-Nde1-6His-Nco1-B

Colony Screening

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Date: 2014_9

Make miniprep, digest with Kpn1 + Mlu1, should see no insert (linearized vector),


Result intepretation/Summary

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Conclusion

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