2331 pT7A31
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Cloning of 2331 pT7A31
[edit]Purpose: Cloning of2331 pT7A31==
Date: 2014-9-15---
Notebook:0
Page:
Clone ID:2331
Experimenters: shanyi
Results
[edit]CLONING
[edit]digest vector with Nde1 + Nco1.
Ligate to annealed oligos 2331-Nde1-6His-Nco1-T + 2331-Nde1-6His-Nco1-B
Colony Screening
[edit]Date: 2014_9
Make miniprep, digest with Kpn1 + Mlu1, should see no insert (linearized vector),
