2345 pCold-HCMVUL98

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Revision as of 13:41, 24 September 2014 by Excellgen (talk | contribs) (Created page with "== '''Cloning of 2345 pCold-HCMVUL98''' == '''Purpose:''' Cloning of2345 pCold-HCMVUL98'''==''' '''Date: '''2014-9---- '''Notebook:0''' '''Page:''' '''Clone ID:2345'' ...")

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Cloning of 2345 pCold-HCMVUL98

Purpose: Cloning of2345 pCold-HCMVUL98==

Date: 2014-9----

Notebook:0

Page:

'Clone ID:2345

Experimenters: shanyi

Results

CLONING

pcr from2344

tagac cat atgtggggcgtTtcgagtttg
2345-HCMVUL98-Xho1-R
(GT ACCACGCCCG GTGAGCCCC ctcgag tagca)
tgctactcgagGGGGCTCACCGGGCGTGGTAC

digest with Nde1 + XhoI

Colony Screening

Date: 2014_8

Make miniprep, use Nde1 + Xho1to find positive clone

DIGEST WITH Nde1 + HindII1

Result intepretation/Summary

Conclusion

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Cloning