2345 pCold-HCMVUL98

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Cloning of 2345 pCold-HCMVUL98

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Purpose: Cloning of2345 pCold-HCMVUL98==

Date: 2014-9----

Notebook:0

Page:

'Clone ID:2345

Experimenters:  shanyi

Results

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CLONING

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pcr from2344

tagac cat atgtggggcgtTtcgagtttg
2345-HCMVUL98-Xho1-R
(GT ACCACGCCCG GTGAGCCCC ctcgag tagca)
tgctactcgagGGGGCTCACCGGGCGTGGTAC


digest with Nde1 + XhoI


Colony Screening

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Date: 2014_8

Make miniprep, use  Nde1 + Xho1to find positive clone


DIGEST WITH Nde1 + HindII1

Result intepretation/Summary

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Conclusion

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