2297 pEC-NotI

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Cloning of 2297 pEC-NotI

Purpose: Cloning of2297 pEC-NotI==

Date: 2014-9

Notebook:0

Page:

Clone ID:2297

Experimenters: shanyi

Results

CLONING

PCR from 567

Not1-Xba1-F atacactctagaTGCGTAGCGATACCAGCGTTG

2297-Not1-HindIII-R2 tgataAAGCTTTCAGTGGTGGTGGTGGTGGTGTGCATCAAACAGACCACGCTG

digest with Xba1 + HindIII, clone to1454 pEC

Colony Screening

Date: 2014_8_8

Make miniprep, use Xba1 + HindIII to find positive clone

Result intepretation/Summary

Conclusion

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Cloning