2297 pEC-NotI
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Cloning of 2297 pEC-NotI
[edit]Purpose: Cloning of2297 pEC-NotI==
Date: 2014-9
Notebook:0
Page:
Clone ID:2297
Experimenters: shanyi
Results
[edit]CLONING
[edit]PCR from 567
Not1-Xba1-F atacactctagaTGCGTAGCGATACCAGCGTTG
2297-Not1-HindIII-R2 tgataAAGCTTTCAGTGGTGGTGGTGGTGGTGTGCATCAAACAGACCACGCTG
digest with Xba1 + HindIII, clone to1454 pEC
Colony Screening
[edit]Date: 2014_8_8
Make miniprep, use Xba1 + HindIII to find positive clone
