2349 pT7A3-Cas9-Nickase

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Cloning of 2349 pT7A3-Cas9-Nickase

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Purpose: Cloning of2349 pT7A3-Cas9-Nickase==

Date: 2014-9----

Notebook:0

Page:

Clone ID:2349

Experimenters:  shanyi

Results

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CLONING

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hCas9-D10A-Nco1-F ctagcccatggacaagaagtactccattgggctcgCtatcggcac

2334-Cas9-BamH1-R ATGGCGGGGGATCCGGCCAGATTG


digest with NCO1 + BamH1, clone to 2332 pT7A30-Cas9



Colony Screening

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Date: 2014_8

Make miniprep, use  Nco1 + BamH1 to find positive clone





Result intepretation/Summary

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Conclusion

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