2349 pT7A3-Cas9-Nickase

From ELN
Jump to navigation Jump to search

Cloning of 2349 pT7A3-Cas9-Nickase

[edit]

Purpose: Cloning of2349 pT7A3-Cas9-Nickase==

Date: 2014-9----

Notebook:0

Page:

Clone ID:2349

Experimenters:  shanyi

Results

[edit]

CLONING

[edit]

hCas9-D10A-Nco1-F ctagcccatggacaagaagtactccattgggctcgCtatcggcac

2334-Cas9-BamH1-R ATGGCGGGGGATCCGGCCAGATTG


digest with NCO1 + BamH1, clone to 2332 pT7A30-Cas9



Colony Screening

[edit]

Date: 2014_8

Make miniprep, use  Nco1 + BamH1 to find positive clone





Result intepretation/Summary

[edit]

Conclusion

[edit]
Retrieved from "https://eln.excellgen.com/index.php?title=2349_pT7A3-Cas9-Nickase&oldid=13374"