1917 pT7A3E-6H-MCviPI

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Cloning of 1917 pT7A3E-6H-MCviPI

Purpose: Cloning of1917 pT7A3E-6H-MCviPI==

Date: 2014-9----

Notebook:0

Page:

Clone ID:1917

Experimenters: shanyi

Results

CLONING

gene synthesis

join two fragments

digest with Nde1 + Sal1,clone to 2021 pT7A3E-6H-HC

Colony Screening

Date: 2014_9

Make miniprep, use Nde1 +XHO1 to find positive clone

1917-5 missing 6nt, need to fix

Result intepretation/Summary

Conclusion

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Cloning