1917 pT7A3E-6H-MCviPI
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Cloning of 1917 pT7A3E-6H-MCviPI
[edit]Purpose: Cloning of1917 pT7A3E-6H-MCviPI==
Date: 2014-9----
Notebook:0
Page:
Clone ID:1917
Experimenters: shanyi
Results
[edit]CLONING
[edit]gene synthesis
join two fragments
digest with Nde1 + Sal1,clone to 2021 pT7A3E-6H-HC
Colony Screening
[edit]Date: 2014_9
Make miniprep, use Nde1 +XHO1 to find positive clone
1917-5 missing 6nt, need to fix