2380 pEC2-Apyrase

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Cloning of2380 pEC2-Apyrase

Purpose: Cloning of2380 pEC2-Apyrase-==

Date: 2014-12----

Notebook:0

Page:

Clone ID:2380

Experimenters: shanyi

Results

CLONING

use 2384-1 as template, fix the missing T

2382-Apyrase-1-1+2382-Apyrase-1-6

2382-Apyrase-1-7+2382-Apyrase-2-18

join two fragments by pcr

Digest with Nde1 + Xho1

Colony Screening

Date: 2014_11

Make miniprep, use Nde1 + Xho1 to find positive clone

Result intepretation/Summary

Conclusion

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Cloning