2380 pEC2-Apyrase

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Cloning of2380 pEC2-Apyrase

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Purpose: Cloning of2380 pEC2-Apyrase-==

Date: 2014-12----

Notebook:0

Page:

Clone ID:2380

Experimenters:  shanyi

Results

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CLONING

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use 2384-1 as template, fix the missing T

2382-Apyrase-1-1+2382-Apyrase-1-6

2382-Apyrase-1-7+2382-Apyrase-2-18


join two fragments by pcr


Digest with Nde1 + Xho1


Colony Screening

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Date: 2014_11

Make miniprep, use Nde1 + Xho1 to find positive clone


Result intepretation/Summary

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Conclusion

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