2325 pET30b-Ecoli-MutL

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Cloning of 2325 pET30b-Ecoli-MutL

Purpose: Cloning of2325 pET30b-Ecoli-MutL==

Date: 2015-1---

Notebook:0

Page:

Clone ID:2325

Experimenters: shanyi

Results

CLONING

PCR from Escherichia coli str. K-12 substr. MG1655 genomic DNA

2325-Ecoli-MutL-Nde1-F tatcacatatgccaattcaggtcttaccgcca

2325-Ecoli MutL-Xho1-R TGGTGctcgagctcgagctcatctttcagggctt

Digest with Nde1 + Xho1

Colony Screening

Date: 2014_9

Make miniprep, use Nde1 + Xho1 to find positive clone

Result intepretation/Summary

Conclusion

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Cloning